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目的 :探讨尾加压素Ⅱ (UII)受体在慢性低氧性右心室肥大中的作用。方法 :在慢性低氧高二氧化碳肺动脉高压、右心室肥大的大鼠模型上 ,采用放射性配基结合法 ,测定不同缺氧时间 ( 2周、4周 )右心室肌浆膜上UII受体的结合率 ,放免法测定血浆UII的含量。结果 :慢性低氧高二氧化碳大鼠的肺动脉平均压 (mPAP)和右心室(RV)与左心室加室间隔 (LV +S)重量比 (RV/LV +S)明显高于对照组 (P <0 0 1) ;UII受体结合位点 (Bmax) ,低氧 2周组比正常对照组高 2 6 7% (P <0 0 1) ,4周组又比 2周组高 19 8% (P <0 0 1) ,UII受体亲和力 (Kd值 ) 3组间无显著差别 (P >0 0 5 ) ;血浆UII水平呈先高后降的双向变化 ,2周组比正常组高 33 5 % (P <0 0 1) ,而 4周组比 2周组低15 4 % (P <0 0 5 ) ,与正常对照组相近。结论 :慢性低氧高二氧化碳使大鼠右心室肌浆膜上UII受体增加 ,其变化可能参与了右室肥大
Objective: To investigate the role of urotensin Ⅱ (UII) receptor in chronic hypoxic right ventricular hypertrophy. Methods: Radioligand binding assay was used to determine the binding of UII receptor in the right ventricular myocardium at different hypoxia (2 weeks, 4 weeks) in a rat model of chronic hypoxia and hypercapnia pulmonary hypertension and right ventricular hypertrophy Rate, radioimmunoassay determination of plasma UII content. Results: The mean pulmonary arterial pressure (mPAP) and RV / LV + S ratio (RV / LV + S) in chronic hypoxic hypercapnic rats were significantly higher than those in control group (P < (P <0.01). The UII receptor binding site (Bmax) was 26.7% (P <0.01) higher in the hypoxemia group than in the normal control group and 19.8% (P < There was no significant difference in UII receptor affinity (Kd) between the three groups (P> 0.05). The level of UII in plasma increased bidirectionally and then decreased, % (P <0.01), while the 4-week group was 15.4% lower than the 2-week group (P <0 05), similar to the normal control group. CONCLUSION: Chronic hypoxia and hypercapnia increase the UII receptor in serous membrane of right ventricular in rats, which may be involved in the changes of right ventricular hypertrophy