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报道了在表面活性剂 Ｔｒｉｔｏｎ Ｘ－１００存在下，利用Ｇｄ（Ⅲ）离子增敏Ｃｅ（Ⅳ）和茶碱氧化产物的固有发光的协同效应，建立了高灵敏度的测定茶碱的荧光分析方法，其荧光光谱的激发波长λｃｘ＝３２５ｎｍ发射波长λｃｍ＝４０４ｎｍ。在０．ｌ～２０ ｍｇ／Ｌ范围内荧光强度与茶碱浓度呈良好线性关系，其检测下限为０．０５ ｍｇ／Ｌ，方法精密度为２．５％，回收率在９６．０％～１０７．１％，用于血清中痕量茶碱分析取得了满意结果。
The synergistic effect of the intrinsic luminescence of Ce (Ⅳ) sensitized with Gd (Ⅲ) ions and the oxidation of theophylline was studied in the presence of the surfactant Triton X-100 to establish a high sensitivity fluorescence analysis method for theophylline determination. Its fluorescence spectrum of the excitation wavelength λcx = 325nm emission wavelength λcm = 404nm. At 0. L ~ 20 mg / L range of fluorescence intensity and theophylline concentration showed a good linear relationship between the detection limit of 0.05 mg / L, the method precision of 2.5%, the recovery rate of 96.0% ~ 107.1 %, For the trace of theophylline in serum analysis has yielded satisfactory results.