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目的:研究内源性神经肽神经降压素(neurotensin,NT)在脊髓背角胶状质(substantia gelatinosa,SG)内对突触前神经递质释放的影响。方法:采用全细胞电压膜片钳记录方法,在脊髓薄片上观察NT对SG内微小兴奋性突触后电流(mEPSCs)和微小抑制性突触后电流(mIPSCs)的频率和幅值的影响。结果:(1)灌流NT(2μmol/L)对SG内神经元mEPSCs的频率和幅值均无明显影响,说明NT不影响SG内兴奋性神经递质的释放;(2)灌流NT(2μmol/L)能增加SG内神经元mIPSCs的频率,但对幅值无明显影响,即NT可引起突触前抑制性神经递质的释放增加,但对突触后神经元无明显影响。结论:NT可通过增加SG内抑制性神经递质释放的途径抑制伤害性信息的传递,从而实现镇痛效应。
Objective: To investigate the effect of neurotensin (NT) on the release of presynaptic neurotransmitter in substantia gelatinosa (SG). Methods: Whole-cell voltage patch-clamp recording was used to observe the effects of NT on the frequency and amplitude of mSPS and mIPSCs in the spinal cord slices. Results: (1) NT (2μmol / L) had no significant effect on the frequency and amplitude of mEPSCs in SG neurons, indicating that NT did not affect the release of excitatory neurotransmitter in SG. (2) NT (2μmol / L) increased the frequency of mIPSCs in SG neurons, but had no significant effect on the amplitude. That is, NT could increase the release of presynaptic inhibitory neurotransmitter, but had no significant effect on post-synaptic neurons. Conclusion: NT can inhibit the transmission of nociceptive information by increasing the release of inhibitory neurotransmitter in SG, thus achieving the analgesic effect.