目的:研究赖氨酸特异性组蛋白去甲基化酶1(lysine specific demethylase 1,LSD1)在人结肠癌组织中的表达,及其对人结肠癌LoVo细胞株转移潜能的影响。方法:收集2007-06-01-2011-08-31南通大学附属医院52例结肠癌石蜡组织标本,免疫组化方法检测52例组织中LSD1的表达情况并分析其临床意义;瞬时转染LSD1特异性短发卡RNA(shRNA)干扰质粒下调LSD1的表达,通过细胞划痕实验、Transwell迁移实验和Transwell侵袭实验观察下调LSD1的表达后LoVo细胞迁移及侵袭行为的变化。结果:LSD1在结肠癌组织中阳性表达率为67.3%(35/52),在癌旁组织中为37.5%(9/24),χ2=5.958,P=0.014。结合临床资料分析显示,LSD1的表达与分化程度(P=0.001)、淋巴结转移(P=0.011)及Duke分期(P=0.024)有关,而与患者性别、年龄、肿瘤部位、肿瘤直径、大体类型、浸润深度及有无远处转移无关。细胞划痕结果显示,转染组细胞迁移能力明显受抑制;Transwell迁移实验结果显示,Control组和转染LSD1-shRNA组穿过膜细胞数分别为220.25±6.75和40.875±1.813,转染LSD1-shRNA组穿过膜细胞数明显减少,z=3.787,P<0.001;Transwell侵袭实验结果显示,Control组和转染LSD1-shRNA组穿过膜细胞数分别为222.75±6.25和33.625±1.75,转染LSD1-shRNA组穿过膜细胞数明显减少,z=3.782,P<0.001。结论:LSD1表达与结肠癌的恶性程度及转移潜能密切相关,下调LSD1表达能抑制结肠癌LoVo细胞的转移潜能。 Objective: To investigate the expression of lysine-specific demethylase 1 (LSD1) in human colon cancer and its effect on the metastatic potential of human colon cancer LoVo cell line. METHODS: Totally 52 paraffin-embedded specimens of colon cancer were collected from Affiliated Hospital of Nantong University from June 2007 to January 2007 with the help of immunohistochemistry. The expression of LSD1 in 52 cases was detected by immunohistochemistry and its clinical significance was analyzed. The expression of LSD1 was downregulated by RNA interference plasmid, and the changes of migration and invasion of LoVo cells were observed by cell scratch assay, Transwell migration assay and Transwell invasion assay. Results: The positive rate of LSD1 in colon cancer tissues was 67.3% (35/52), in para-cancerous tissues 37.5% (9/24), χ2 = 5.958, P = 0.014. Combined with clinical data analysis, the expression of LSD1 was correlated with the degree of differentiation (P = 0.001), lymph node metastasis (P = 0.011) and Duke staging (P = 0.024), but not with gender, age, tumor location, tumor diameter, gross type , Infiltration depth and whether the distant metastasis has nothing to do. The results of cell scratch assay showed that the migration ability of transfected cells was significantly inhibited. The results of Transwell migration assay showed that the numbers of cells passing through the control and transfected LSD1-shRNA groups were 220.25 ± 6.75 and 40.875 ± 1.813, respectively. The transfected LSD1- The number of transfected cells in shRNA group was significantly decreased (z = 3.787, P <0.001). Transwell invasion assay showed that the number of cells passing through the membrane was 222.75 ± 6.25 and 33.625 ± 1.75 respectively in Control group and LSD1-shRNA transfected group, LSD1-shRNA group significantly decreased the number of cells across the membrane, z = 3.782, P <0.001. Conclusion: The expression of LSD1 is closely related to the malignant degree and metastatic potential of colon cancer. Down-regulation of LSD1 expression can inhibit the metastatic potential of colon cancer LoVo cells.