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本文证明兔肌线粒体甘油-3-磷酸脱氢酶(E.C.1.1.99.5)经Triton X-100增溶、羟基磷灰石与DEAE-Sepharose CL6B柱层析和蔗糖密度梯度超离心,得到电泳纯的酶制剂,比活力提高200倍,总活力回收为10％。酶蛋白多肽链的表现分子量为69000。酶-TritonX-100复合物的Stockes′半径为59(?)。沉降系数为10.7s。每毫克蛋白含1.7mg TritonX-100和26μg磷脂。酶在410nm和460nm处有两个吸收峰,分别为非血红素铁和FAD。底物甘油-3-磷酸可大大降低这两个吸收峰值。
This paper demonstrates that rabbit muscle mitochondrial glycerol-3-phosphate dehydrogenase (EC1.1.99.5) was solubilized by Triton X-100, purified by hydroxyapatite and DEAE-Sepharose CL6B column chromatography and sucrose density gradient ultracentrifugation to obtain electrophoretic pure Enzyme preparation, 200 times more specific activity, the total recovery of 10% of vitality. Enzyme protein polypeptide chain performance molecular weight of 69,000. The Stockes’ radius of the enzyme -Triton X-100 complex is 59 (?). Sedimentation coefficient is 10.7s. 1.7 mg TritonX-100 per mg protein and 26 μg phospholipids. The enzyme has two absorption peaks at 410 nm and 460 nm, non-heme iron and FAD, respectively. Substrate glycerol-3-phosphate can greatly reduce these two absorption peaks.