合成了新试剂 1 ,5-二 (2 -羟基 - 5-溴苯 ) - 3-氰基甲月替 (HBPCF) ,并研究了它与锌的显色反应。在 p H 9.5的氨 - NH4 Cl介质中 ,Zn( )与 HBPCF反应生成 1∶ 2的蓝色配合物 ,在波长660 nm处有最大吸收 ,表观摩尔吸光系数为 2 .9× 1 0 4 。 Zn( )浓度在 0～ 2 5μg/2 5ml服从比耳定律。方法应用于头发、葡萄糖营养素和黑米羹中微量锌的直接测定 ,其准确性和重现性较好 A new reagent, 1, 5 - bis (2 - hydroxy - 5 - bromophenyl) -3 - cyanomethylene replacement (HBPCF) was synthesized and its color reaction with zinc was studied. In ammonia-NH4Cl medium of p H 9.5, Zn () reacted with HBPCF to form a blue complex of 1: 2 and had the maximum absorption at a wavelength of 660 nm with an apparent molar absorptivity of 2.9 × 10 4 . The concentration of Zn () 0 ~ 25μg / 2 5ml obey Beer’s law. The method was applied to the direct determination of trace zinc in hair, glucose and black rice soup, and its accuracy and reproducibility were better