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近十几年来，缺血性脑损伤的细胞分子机制已成为神经科学界的重要研究内容之一。本实验用免疫组织化学定量分析方法对四管阻塞造成的全脑缺血20min后再灌流0～24h的大鼠海马小白蛋白中间神经元的变化进行了观察。结果显示：再灌流0～6h之间CA2、CA3区小白蛋白阳性中间神经元均出现一过性减少．到24h时恢复三缺血前水平：而CA1区小白蛋白阳性中间神经元的减少出现于再灌流12h后。此外齿状回颗粒层及门区小白蛋白阳性中间神经元变化不显著。本实验结果提示，短暂性全脑缺血早期海马各区域小白蛋白阳性神经元减少的不同时相特征．可能与海马各区域的不同缺血敏感性及不同缺血的病理学表现有关。
In recent decades, the cellular and molecular mechanisms of ischemic brain injury have become one of the important research topics in the neuroscience community. The experiment using immunohistochemical quantitative analysis of four-tube occlusion caused by global ischemia in rats after reperfusion 0 ~ 24h rat white albumin intermediate neurons changes were observed 20 min after global cerebral ischemia. The results showed that there was a transient decrease of albumin positive interneurons in CA2 and CA3 between 0 and 6 h after reperfusion. The levels of pre-ischemic ischemia were restored by 24 h: the reduction of small albumin-positive interneurons in CA1 zone occurred 12 h after reperfusion. In addition, the dentate gyrus granule layer and portal albumin positive interneurons did not change significantly. The results of this experiment suggest that there are different phases of small albumin positive neurons in the hippocampus in the early stage of transient global cerebral ischemia. May be related to the different ischemic sensitivity of different regions of the hippocampus and pathological manifestations of different ischemia.