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目的 :用Ames试验、小鼠骨髓嗜多染红细胞微核试验、人体外周血淋巴细胞姐妹染色单体交换 (SCE)试验以及小鼠精子畸形试验对重质芳烃进行了致突变性研究。结果 :重质芳烃无移码突变和碱基置换效应 ,微核试验显示在 316～ 15 80mg/kg·bw剂量范围小鼠骨髓嗜多染红细胞微核率显著增加 (与阴性对照组比较P <0 .0 1) ;SCE试验结果显示在活化系统 (S9混合液 )存在的条件下 ,人外周血淋巴细胞SCE频率较对照组显著增高 (在 0 .5mg/ml及 5mg/ml剂量组 ) ;小鼠精子畸形试验显示在 15 80mg/kg·bw剂量水平小鼠精子畸形率显著增高 ,与阴性对照组比较差异有显著性 (P <0 .0 1)。结论 :重质芳烃具有间接致突变性 ,对其职业性接触及应用的安全性应给予高度重视。
OBJECTIVE: To study the mutagenicity of heavy aromatics with Ames test, micronucleus test of mouse bone marrow polychromatic erythrocytes, sister chromatid exchange (SCE) test of human peripheral blood lymphocytes and sperm abnormality test in mice. Results: There was no frameshift mutation and base substitution in heavy aromatics. The micronucleus test showed that the micronucleus rate of bone marrow polychromatic erythrocytes increased significantly in the range of 316-1580 mg / kg · bw (P < 0. 01). The results of SCE showed that the SCE frequency of human peripheral blood lymphocytes was significantly higher than that of the control group (at 0.5 mg / ml and 5 mg / ml) in the presence of activated system (S9 mixture) Mice sperm deformity test showed that the sperm deformity rate of mice at the dose of 15 80mg / kg · bw was significantly higher than that of the negative control group (P <0.01). Conclusion: The heavy aromatics have indirect mutagenicity, and their occupational exposure and application safety should be given high priority.