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1引言 盐酸黄连素是一种用于治疗肠道感染,毒性低、副作用小的天然药物。对盐酸黄连素的测定,化学发光法未见报道。本文发现,过氧化氢存在下,磷酸介质中盐酸黄连素与高碘酸钠反应产生强的化学发光。据此结合流动注射技术建立了在NaIO4-H3PO4-H2O2-盐酸黄
1 Introduction Berberine hydrochloride is a natural medicine used to treat intestinal infections with low toxicity and small side effects. Determination of berberine hydrochloride, chemiluminescence method has not been reported. This paper found that, in the presence of hydrogen peroxide, the reaction of berberine hydrochloride and sodium periodate in the phosphoric acid medium produces strong chemiluminescence. Based on this, flow cytometry was used to establish NaIO4-H3PO4-H2O2-HCl yellow