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目的 探讨柯萨奇病毒 Ｂ 基因及特性抗体测定在病毒性心肌炎诊断中的临床意义。方法 用 Ｐ Ｃ Ｒ 及 Ｅ Ｌ Ｉ Ｓ Ａ 法检测柯萨奇病毒 Ｂ。结果 ４０ 例心肌炎患儿用 Ｐ Ｃ Ｒ 方法检出１７ 例柯萨奇病毒 Ｂ 基因，对照组无一例阳性；４０ 例患儿柯萨奇病毒 Ｂ 特异性抗体１４ 例阳性，正常对照有２ 例阳性。发病短于１ 个月者２９ 例中，１３ 例柯萨奇病毒基因阳性，柯萨奇病毒 Ｂ 特异性抗体阳性４ 例。结论 利用 Ｐ Ｃ Ｒ 方法检测柯萨奇病毒 Ｂ 具有阳性率高，特异性强的特点。
Objective To investigate the clinical significance of Coxsackie virus B gene and antibody in the diagnosis of viral myocarditis. Methods Coxsackie virus B was detected by P C R and E L I S A method. Results In 40 children with myocarditis, 17 Coxsackievirus B genes were detected by P C R method, and none was found in the control group. Fourteen children with Coxsackie B virus were positive in the control group and 2 were positive in the normal control group . In 29 cases with onset less than 1 month, 13 Coxsackievirus positive and Coxsackievirus B were positive in 4 cases. Conclusion The detection of Coxsackievirus B by P C R has the characteristics of high positive rate and strong specificity.