为分离糖蛋白的微观不均一组分 ,建立了以醇胺为电泳缓冲液主要成分、以聚丙烯酰胺涂层毛细管为分离通道的电泳方法 .利用本法可使琼脂糖电泳纯的鸡卵白蛋白分裂出 2 5个以上的峰 ,使转铁蛋白分裂出 1 0个左右的峰 .控制分离能力的关键因素包括缓冲液的组成、浓度、pH和毛细管内壁的性质等 ,其选择与样品有关 . In order to separate the microscopic heterogeneous components of glycoproteins, an electrophoresis method was established to use alcohol amines as the main component of the electrophoresis buffer and polyacrylamide coated capillaries as the separation channel. By using this method, agarose electrophoresis pure chicken egg albumin Cleavage of more than 25 peaks, the transferrin split about 10 peaks.The key factors to control the separation capacity, including the composition of the buffer, concentration, pH and the nature of the capillary wall, the choice of the sample.