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本文在人工气候室中，用营养液培养方法，并结合同位素示踪技术研究了铁的供应状况、两种形态氮素（NO3--N和NH4+-N）及叶片遮光对菜豆体内铁再利用效率的影响，并对其有关机理进行了深入的研究。结果表明，铁的缺乏有利于累积在根和初生叶中的铁向新生组织中转移，铁的再利用效率明显提高。无论在缺铁还是供铁条件下，NH4+-N的供应使得菜豆新叶中活性铁含量、新叶叶绿素含量及体内铁的再利用效率都明显高于NO3--N处理，叶绿体超微结构完整，井且新叶未出现黄化现象；特别是在处理后期，差异更加明显。初生叶遮光使得新叶中活性铁含量增加，体内铁的再利用效率提高了6个百分点左右，在一定程度上改善了NO3－－N营养缺铁菜豆新叶的铁营养状况。可见，NH4+-N的供应和叶片遮光都可提高菜豆体内铁的再利用效率，明显改善菜豆新叶的铁营养状况。
In this paper, we use nutrient solution culture method and isotope tracing technique to study the supply status of iron in the artificial climate chamber. Two forms of nitrogen (NO3 - N and NH4 + -N) Efficiency, and its related mechanisms in-depth study. The results showed that the lack of iron is conducive to the accumulation of iron accumulated in the roots and primary leaves to the new tissue transfer, iron reutilization efficiency increased significantly. In both iron deficiency and iron supply conditions, the supply of NH4 + -N led to a significant increase in active iron content, new leaf chlorophyll content and iron recycle efficiency in new leaves of N. tabacum L. compared with NO3 - N treatment. The chloroplast ultrastructure was intact , And the new leaves did not appear yellow phenomenon; especially in the late treatment, the difference is even more obvious. The shading of newborn leaves increased the content of active iron in new leaves, and the iron reuse efficiency in vivo increased about 6%, which improved the iron nutrition status of NO3 - N nutrient deficient new bean. It can be seen that both the supply of NH4 + -N and the shading of leaves can increase the iron recycle efficiency of cabbage bean and obviously improve the iron nutrition status of the new leaf of bean kidney.