Objective: To investigate the association between breastfeeding and infant acquired infection of Hepatitis C Virus(HCV). Methods: To obtain studies eligible for META-analysis, China biological medicine discs(CBMD) and MEDLINE citations were surveyed. Criteria for enrollment of published studies for META-analysis were based on principle by Abdolmaleky HM[1]. Odd ratio(OR) was calculated and summarized by fixed effect model or random effects using RevMan software. The heterogeneity of ORs was assessed using an χ2 test of goodness of fit. The significance of the pooled OR was determined by the z-test. The strength of association was summarized usingOR. An OR>1.0 indicated a positive association between the risk factor and infants HCV infection. Results:After searching in Medline and CBMD, 120 articles were enrolled for further identification. Thirty-seven were of review on relative fields and were excluded. Abstracts of remaining articles were carefully read, and finally, only 6 articles met, the standards for enrollment criteria. After analysis of the factor conced, no significant association was found between breastfeeding and infant HCV infection, with a pooled OR equal to 0.60(95%CI = 0.22-1.60), and proved not to be of risk factors on infant acquired infection of HCV. Conclusion: Breastfeeding dose not increase the risk of infant acquired infection of HCV.