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目的 探讨糖尿病 (DM)患者血浆白细胞介素 18(IL 18)水平及外周血单个核细胞 (PBMCs)IL 18mRNA表达量变化。方法 选取 10名健康志愿者及 30例DM患者 (Ⅱ型糖尿病 15例 ,Ⅰ型糖尿病 15例 )。应用IL 18特异的酶联免疫吸附法(ELISA)测定IL 18血浆水平 ,同时采用半定量逆转录多聚酶链反应 (RT PCR)技术 ,分析PBMCsIL 18mRNA表达量。结果 Ⅱ型糖尿病患者血浆IL 18水平及PBMCsIL 18mRNA表达量与正常对照组差异无统计学意义 [(2 73.1± 99.4 6 )vs (2 4 8.86± 82 .6 8)and(0 .731± 0 .12 9)vs (0 .715± 0 .116 ) ,P >0 .0 5 ];Ⅰ型糖尿病患者血浆IL 18水平及PBMCsIL 18mRNA表达量较正常对照组增高 ,差异有显著统计学意义 [(36 0 .17± 12 5 .4 8)vs (2 4 8.86± 82 .6 8)and (0 .935± 0 .132 )vs (0 .715± 0 .116 ) ,P <0 .0 5 ]。结论 Ⅰ型糖尿病患者外周血IL 18的高表达 ,提示其可能介导本病发病过程 ;IL 18可能不参与Ⅱ型糖尿病的发病过程
Objective To investigate the levels of plasma interleukin 18 (IL 18) and IL 18 mRNA in peripheral blood mononuclear cells (PBMCs) in patients with diabetes mellitus (DM). Methods Ten healthy volunteers and 30 DM patients (15 cases of type II diabetes mellitus and 15 cases of type I diabetes mellitus) were selected. IL 18-specific ELISA was used to determine the plasma levels of IL-18, and the expression of IL-18 mRNA in PBMCs was analyzed by semi-quantitative reverse transcriptase-polymerase chain reaction (RT PCR). Results There was no significant difference between the level of IL-18 and the level of IL-18 mRNA in PBMCs in type 2 diabetes mellitus (2 73.1 ± 99.4 6 vs 24.76 ± 82.68 and 0.73 ± 0.81, respectively) The level of IL-18 in plasma and the level of IL-18 mRNA in PBMCs in type 1 diabetic patients were significantly higher than those in normal controls [(36) ± (9) ± 0.116, P> 0.05] 0 .17 ± 12 5 .4 8) vs (24.886 ± 82.68) and (0.935 ± 0.132) vs (0.715 ± 0.116), P <0.05. Conclusion The high expression of IL 18 in peripheral blood of patients with type 1 diabetes mellitus suggests that it may mediate the pathogenesis of this disease; IL 18 may not participate in the pathogenesis of type 2 diabetes