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目的探讨辅酶Q_(10)对Wistar大鼠主动脉粥样硬化斑块病变程度和斑块内胶原含量的影响。方法 38只Wistar大鼠随机选7只为对照组,给予普通饲料;余31只大鼠给予高脂饲料+维生素D3腹腔注射+卵清白蛋白腹腔注射制备动脉粥样硬化模型,13周后随机选取3只大鼠行主动脉组织病理检查HE染色,动脉粥样硬化斑块形成为造模成功。造模成功后将余28只大鼠随机分为动脉粥样硬化模型组(模型组)、辅酶Q_(10)治疗组(辅酶Q_(10)组)、阿托伐他汀治疗组(阿托伐他汀组)、辅酶Q_(10)+阿托伐他汀治疗组(联合组),每组各7只。对照组和模型组给予等体积生理盐水,其余3组分别给予辅酶Q_(10)30mg/(kg·d),阿托伐他汀5mg/(kg·d),辅酶Q_(10)30mg/(kg·d)+阿托伐他汀5mg/(kg·d),连续8周。取主动脉弓行HE染色和Masson染色,运用计算机图像分析仪检测各组斑块面积、胶原面积,血管横截面积,计算校正斑块面积(斑块面积/血管横截面积)与校正胶原面积(胶原面积/血管横截面积)。结果联合组主动脉弓校正斑块面积为(0.04±0.01),低于阿托伐他汀组(0.10±0.02)、辅酶Q_(10)组(0.09±0.02)和模型组(0.17±0.05)(P<0.05),辅酶Q_(10)组和阿托伐他汀组低于模型组(P<0.05),辅酶Q_(10)组与阿托伐他汀组比较差异无统计学意义(P>0.05);联合组主动脉弓校正胶原面积为(0.33±0.03),高于阿托伐他汀组(0.27±0.03)、辅酶Q_(10)组(0.29±0.04)和模型组(0.18±0.03)(P<0.05),辅酶Q_(10)组和阿托伐他汀组高于模型组(P<0.05),辅酶Q_(10)组与阿托伐他汀组比较差异无统计学意义(P>0.05)。结论辅酶Q_(10)可减轻动脉粥样硬化病变程度,增加斑块内胶原含量而增强斑块稳定性。
Objective To investigate the effect of coenzyme Q_ (10) on the severity of atherosclerotic plaque lesions and plaque collagen content in Wistar rats. Methods Thirty-eight Wistar rats were randomly selected as the control group and fed with normal diet. The remaining 31 rats were given high-fat diet + vitamin D3 intraperitoneal injection + ovalbumin intraperitoneal injection to prepare atherosclerosis model. After 13 weeks of random selection Three rats underwent aortic pathological examination HE staining, atherosclerotic plaque formation for the modeling success. After successful modeling, 28 rats were randomly divided into atherosclerosis model group (model group), coenzyme Q_ (10) treatment group (coenzyme Q_ (10) group), atorvastatin treatment group Statin group), coenzyme Q_ (10) + atorvastatin treatment group (combination group), 7 rats in each group. The control group and model group were given equal volume of normal saline, and the other three groups were given coenzyme Q 10 mg / (kg · d), atorvastatin 5 mg / (kg · d), coenzyme Q 10 mg / kg · D) + atorvastatin 5 mg / (kg · d) for 8 weeks. The aortic arch was examined by HE staining and Masson staining. The plaque area, collagen area and vascular cross-sectional area of each group were measured by computer image analyzer. The corrected plaque area (plaque area / blood vessel cross-sectional area) and corrected collagen area Area / blood vessel cross-sectional area). Results The plaque area of aortic arch in combined group was (0.04 ± 0.01) less than that in atorvastatin group (0.10 ± 0.02), coenzyme Q_ (10) group (0.09 ± 0.02) and model group (0.17 ± 0.05) 0.05), coenzyme Q_ (10) group and atorvastatin group were lower than the model group (P <0.05), coenzyme Q_ (10) group and atorvastatin group was no significant difference (P> 0.05) The corrected collagen area in aortic arch was (0.33 ± 0.03), higher than that in atorvastatin group (0.27 ± 0.03), coenzyme Q_ (10) group (0.29 ± 0.04) and model group (0.18 ± 0.03) Coenzyme Q_ (10) group and atorvastatin group were higher than those in model group (P <0.05). There was no significant difference between coenzyme Q_ (10) group and atorvastatin group (P> 0.05). Conclusions Coenzyme Q_ (10) can reduce the degree of atherosclerosis, increase the collagen content in plaque and enhance the plaque stability.