目的　研究鸟苷酸环化酶抑制药亚甲蓝对感染性休克犬肠道灌注和氧合的影响。方法　静脉注入内毒素诱导的 7只感染性休克犬模型 ,输注 0 9%氯化钠复苏后 15min内注入亚甲蓝 2mg/kg。血流量仪测定基础、休克 1h后、复苏后和亚甲蓝注入后 3 0min肠系膜上动脉血流量。分析动脉和肠系膜上静脉血气 ,计算肠道氧合。结果　犬感染性上动脉 (肠道 )血流减少 61 8% (P<0 0 1) ,氧输送 (DO2 )减少 62 1% (P <0 0 1) ,氧摄取 (O2 extr)增加 2 3 5 %。复苏至肺动脉嵌压为 ( 12 1± 1 4)mmHg后 ,肠道血流增加 5 4 6% (P <0 0 1) ,DO2 增加 12 8% (P <0 0 1) ,O2 extr增加 2 0 9%。亚甲蓝注入后 ,肠道血流增加 19 4% (P <0 0 1) ,DO2 则无明显变化 ( P >0 0 5 ) ,O2 extr增加 14 8%。结论　感染性休克后肠道血流灌注减少 ,氧耗增加 ;液体复苏仅部分恢复肠道灌注 ;亚甲蓝增加肠道灌注和氧摄取 Objective To investigate the effect of guanylate cyclase inhibitor methylene blue on intestinal perfusion and oxygenation in septic shock dogs. Methods Seven rabbits with septic shock induced by endotoxin were injected intravenously with methylene blue 2 mg / kg within 15 minutes after the infusion of 0.9% sodium chloride. Blood flow meter based on the determination, 1h after shock, recovery and methylene blue injection 30min after superior mesenteric artery blood flow. Analysis of arterial and superior mesenteric vein blood gas, calculate intestinal oxygenation. Results The decrease of blood flow in the canine infectious upper gastrointestinal tract was 61 8% (P 0 01), 62% (P 0 01) of oxygen delivery (D 0 2) and 23% (2 02) 5%. After resuscitation to (12 1 ± 14) mmHg, the intestinal blood flow increased by 54.6% (P <0.01), DO2 increased by 12.8% (P <0.01) and O2 extr increased by 2 0 9%. After infusion of methylene blue, intestinal blood flow increased by 19.4% (P <0.01), DO2 showed no significant change (P> 0.05), and O2 extr increased by 14.8%. Conclusions Septic shock reduces intestinal perfusion and increases oxygen consumption. Liquid resuscitation restores intestinal perfusion only partially. Methylene blue increases intestinal perfusion and oxygen uptake