从猪小肠制备碱性磷酸酶,再用戊二醛一步法结合羊抗人IgG;该酶结合物的最高滴度可达1:6 400.以日本血吸虫虫卵粗抗原包板,检测确诊血吸虫病病人血清54份,符合率为100％。测定血吸虫病病人、正常人、小学生、肺吸虫病病人、华支睾吸虫病病人、姜片虫病病人和丝虫病病人血清的阳性率分别为96.5％(222/230)、2.1％(6/280)、0％(0/212)、2.8％(1/35)、5.1％(5/98)、0％(0/5)和6.3％(5/79),表明该酶结合物用于ELISA诊断日本血吸虫病具有较高的敏感性和一定的特异性。 Preparation of alkaline phosphatase from porcine intestine, and then using a one-step glutaraldehyde goat anti-human IgG; the highest titer of the enzyme conjugate up to 1: 6 400. With Schistosoma japonicum egg rough antigen package board, detection of Schistosoma japonicum Sick patient serum 54, in line with the rate of 100%. The positive rates of sera from patients with schistosomiasis, normal people, primary school students, paragonimiasis, Clonorchis sinensis, zingiberaceae and filariasis patients were 96.5% (222/230), 2.1% (6) /280),0%(0/212),2.8%(1/35),5.1%(5/98),0%(0/5) and 6.3% (5/79), indicating that the enzyme conjugate is used ELISA in the diagnosis of schistosomiasis japonica has a high sensitivity and a certain specificity.