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研究建立了超高效液相色谱-质谱联用(LC-MS)法对水果蔬菜中的6种有机酸(酒石酸、苹果酸、柠檬酸、琥珀酸、富马酸和马来酸)进行检测。选用ACQUITY UPLC®HSS T3色谱柱进行分离,0.1%甲酸/乙腈溶液-0.1%甲酸/水体系作为流动相进行梯度洗脱,流速为0.25 m L/min,选择ESI-多反应监测模式进行扫描,确定离子对的质荷比,优化锥孔电压和碰撞电压等质谱条件。通过绘制有机酸的标准曲线,得到富马酸和马来酸的线性范围是0~1000 ng/m L,柠檬酸、酒石酸、琥珀酸、苹果酸的线性范围是0~2000 ng/m L。相关系数R2为0.9989~0.9998之间。最低检出限分别为0.02、0.05μg/m L,最低定量限分别为0.1、0.25μg/m L。
A series of six organic acids (tartaric acid, malic acid, citric acid, succinic acid, fumaric acid and maleic acid) were detected in fruits and vegetables by ultra performance liquid chromatography-mass spectrometry (LC-MS) The separation was performed on an ACQUITY UPLC® HSS T3 column and the gradient elution was carried out using 0.1% formic acid / acetonitrile-0.1% formic acid / water as the mobile phase at a flow rate of 0.25 mL / min. The ESI- Determine the mass-to-charge ratio of the ion pair, and optimize the mass spectrometry conditions such as the cone voltage and the collision voltage. The linear range of fumaric acid and maleic acid was 0-1000 ng / m L by plotting the standard curve of organic acids. The linear range of citric acid, tartaric acid, succinic acid and malic acid was 0-2000 ng / m L. The correlation coefficient R2 is between 0.9989 and 0.9998. The minimum detection limit were 0.02,0.05μg / m L, the minimum limit of quantification was 0.1,0.25μg / m L.