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黄体期的精确评价需要多次取静脉血样测定孕酮,并了解子宫内膜活检标本是否符合时相。多次取样是不切实际的,但如减少取样次数则有损结果的准确性。取唾液标本测定孕酮是可行的,而且可作为游离未结合孕酮的参数。 33例有规则月经周期的妇女,自基温升高第一天起,每三天取静脉血浆和唾液标本一次。每个受试者取子宫内膜活检的时间相当于排卵后10～14天(根据下次月经推算)。用标准放射免疫测定技术测定血浆孕酮。唾液经离心、过滤,用乙醚提取,应用一种特异性抗体,经放射免疫测定法定量。
Precise evaluation of luteal phase requires multiple venous blood samples to determine progesterone, and to understand whether endometrial biopsies are in phase. Multiple sampling is impractical, but decreasing the number of samples compromises the accuracy of the results. Determination of saliva samples of progesterone is feasible, and can be used as free unbound progesterone parameters. In 33 women with regular menstrual cycles, venous plasma and saliva samples were taken every third day starting on the first day of their temperature rise. Each subject took the endometrial biopsy time equivalent to 10 to 14 days after ovulation (based on the next menstruation projections). Plasma progesterone was measured using standard radioimmunoassay techniques. Saliva was centrifuged, filtered and extracted with ether. A specific antibody was used and quantified by radioimmunoassay.