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目的探索低氧干预对实验性糖尿病大鼠血糖及其病症的影响。方法采用长期高糖高脂膳食加小剂量链脲佐菌素(STZ)的方法建立糖尿病大鼠模型,将复制成功的模型大鼠随机分为模型对照组与低氧干预组。模型对照组在常氧环境下正常生活,低氧干预组大鼠每天在低氧帐篷内低氧暴露1 h(O2浓度15.4%±0.2%)、每周休息1 d、共干预4周。结果 (1)糖尿病大鼠出现了典型的“三多一少”特征。(2)通过4周实验干预,低氧干预组大鼠血糖稍高于模型对照组(P﹥0.05)、摄食量明显低于模型对照组(P﹤0.05)、体重下降显著大于模型对照组(P﹤0.05)、肥胖指数、饮水量、胰岛素敏感指数及胰岛素抵抗指数与模型对照组相比差异均无统计学意义(P﹥0.05)。结论持续4周每天1h低氧(O2浓度15.4%±0.2%)暴露对实验性糖尿病大鼠病症无明显改善作用,而体重下降更快、空腹血糖有升高趋势。
Objective To explore the effect of hypoxia on blood glucose and its symptoms in experimental diabetic rats. Methods Long-term high-sugar and high-fat diet plus low-dose streptozotocin (STZ) was used to establish a diabetic rat model. The successful model rats were randomly divided into model control group and hypoxia intervention group. The model control group was normal living under normoxia. The rats in hypoxia intervention group were exposed to hypoxia for 1 h every day for 1 h (O2 concentration 15.4% ± 0.2%) and 1 week rest for 4 weeks. Results (1) Diabetic rats showed the typical characteristics of “three more and one less”. (2) After 4 weeks of intervention, the blood glucose of rats in hypoxia intervention group was slightly higher than that of model control group (P <0.05), the food intake was significantly lower than that of model control group (P <0.05), and the weight loss was significantly greater than that of model control group P <0.05). There was no significant difference in obesity index, water intake, insulin sensitivity index and insulin resistance index between the model control group and the control group (P> 0.05). Conclusion Exposure to hypoxia (15.4% ± 0.2% O2) for 1 h each day for 4 weeks did not improve the condition of experimental diabetic rats, but the weight loss was faster and the fasting blood glucose increased.