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香草醛酸性试剂比色法是检测儿茶素含量最常见方法,但其准确性及实用性一直受到质疑。利用溶剂萃取、TLC和HPLC等方法,对儿茶素-香草醛酸性试剂显色方法的特异性、准确性进行了研究。结果显示,儿茶素类显色反应的灵敏度远高于黄酮醇、二氢黄酮醇和茶皂素类,又由于这些物质在茶叶中的含量很低,因此不会干扰儿茶素总量的测定。通过对显色试剂中酸的种类、酸浓度、显色温度和显色时间的研究,得出儿茶素检测的最佳方法:采用1%香草醛-浓盐酸试剂,在室温下反应5min,以Y(A505)=aX+b公式计算茶树中儿茶素的总含量。
Vanillin acid reagent colorimetric method is the most common method of detection of catechin, but its accuracy and practicability has been questioned. The specificity and accuracy of the method for color development of catechin-vanillic acid reagent were studied by solvent extraction, TLC and HPLC. The results showed that the sensitivity of the color reaction of catechins is much higher than that of flavonols, dihydroflavonols and tea saponins, and because these substances are very low in tea, they do not interfere with the determination of the total amount of catechins . Through the study of acid type, acid concentration, color temperature and color development time, the best method of catechin detection was obtained: using 1% vanillin - concentrated hydrochloric acid reagent, reacting at room temperature for 5 min, The total content of catechins in tea tree was calculated with Y (A505) = aX + b formula.