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目的观察聚苯乙烯纳米粒对人肾小管上皮细胞(HK-2)内活性氧水平的影响。方法用不同浓度的PS、氨基聚苯乙烯(PS-NH2)及羧基聚苯乙烯(PS-COOH)纳米粒刺激HK-2细胞0.5 h,经DCFH-DA染色后,用流式细胞仪检测其平均荧光强度。结果 0.1μg·m L~(-1)PS纳米粒可引起细胞内的活性氧增加,2、4、100μg·m L~(-1)PS纳米粒可引起活性氧降低;4、100μg·m L~(-1)PS-NH_2纳米粒可引起细胞内活性氧降低;0.1、1、2、4、100μg·m L~(-1)PS-COOH纳米粒可引起细胞内活性氧降低。结论低浓度PS纳米粒可增加HK-2细胞内的活性氧水平,高浓度则减少活性氧;PS-COOH纳米粒的活性氧降低效应比PS-NH_2强。
Objective To observe the effect of polystyrene nanoparticles on reactive oxygen species in human renal tubular epithelial cells (HK-2). Methods HK-2 cells were stimulated with different concentrations of PS, PS-NH2 and PS-COOH nanoparticles for 0.5 h. After DCFH-DA staining, the cells were detected by flow cytometry Average fluorescence intensity. Results 0.1μg · m L -1 PS nanoparticle induced an increase of intracellular reactive oxygen species (ROS), while 2,4,100 μg · mL -1 PS nanoparticle decreased reactive oxygen species (ROS); 4,100 μg · m L ~ (-1) PS-NH2 nanoparticles could reduce the intracellular reactive oxygen species (ROS); 0.1,1,2,4,100μg · m L -1 PS-COOH NPs could reduce intracellular reactive oxygen species. CONCLUSIONS: Low concentrations of PS nanoparticles can increase reactive oxygen species in HK-2 cells and decrease reactive oxygen species in high concentrations. PS-COOH nanoparticles have stronger reactive oxygen species reduction than PS-NH_2.