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本文介绍了制备单克隆抗HBs—抗独特型(抗HBs—抗Id)抗体的方法,并用其作为刺激物,在体外刺激人B淋巴细胞,结果证明:用兔抗HBs—Fab和抗HBs—抗Id确可明显提高B淋巴细胞增殖、分泌抗体的能力,而且二者的刺激作用远超过纯化的HBsAg作用。
This article describes a method for preparing monoclonal anti-HBs-anti-idiotypic (anti-HBs-anti-Id) antibody and uses it as a stimulator to stimulate human B lymphocytes in vitro. The results demonstrate that using rabbit anti-HBs- Anti-Id can significantly improve the ability of B lymphocytes to proliferate and secrete antibodies, and the stimulating effects of both greatly exceed the effect of purified HBsAg.