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目的 :比较人脐血 (CB)和外周血 (PB)单个核细胞 (MCs)的增生活性及其紫外线 B照射 (UVB)在增生活性上的抑制作用。方法 :应用 PHA诱导细胞增生以 3 H- Td R结合检测增生活性 (cpm) ;应用 CBMCs和PBMCs的单向混合淋巴细胞培养 (ML C)测试各自的增生反应 (cpm) ,UVB照射后的 PHA刺激和 ML C抑制作用 ,以未照射细胞的活性百分数表示。结果 :人 CBMCs和 PBMCs cpm平均值无统计学差异 ,但单个实验的标准偏差均显示人 CBMCs的 cpm高于 PBMCs,二者对小剂量 U VB照射均十分敏感 ,CBMCs经 1.0 m J/ cm2～ 2 .0 m J/cm2 U VB照射后 ,PHA刺激和单向 ML C分别下降 5 0 %以下及 2 5 .6 %以下。结论 :CBMCs和 PBMCs不仅有类似反应 ,有可能 CBMCs比 PBMCs有更强的增生反应 ,因此 CB移植需预防 GVHD,U VB照射可明显灭活 CBMCs和PBMCs的增生反应 ,U VB照射可能是更为简便、敏感、安全、有效的体外预防 GVHD的方法 ,可能更适合 CB移植。
OBJECTIVE: To compare the proliferative activity of umbilical cord blood (CB) and peripheral blood mononuclear cells (PBs) and the inhibitory effect of UVB irradiation on proliferative activity. METHODS: Proliferative activity (cpm) was measured by 3H-TdR binding assay using PHA-induced cell proliferation. The proliferative response (cpm) was measured by unidirectional mixed lymphocyte culture (MLC) using CBMCs and PBMCs. PHA after UVB irradiation Stimulation and ML C inhibition were expressed as percent of non-irradiated cells. Results: There was no significant difference in mean cpm between human CBMCs and PBMCs. However, the standard deviation of individual experiments showed that the cpm of human CBMCs was higher than that of PBMCs. Both of them were sensitive to low dose UBV irradiation. 2 .0 m J / cm2 U VB, PHA stimulation and unidirectional ML C decreased below 50% and below 25.6%. CONCLUSION: CBMCs and PBMCs not only have similar responses, but CBMCs may have stronger proliferative responses than PBMCs. Therefore, GVHD should be prevented in CB transplantation. UBV irradiation can obviously inactivate the proliferative responses of CBMCs and PBMCs. UBV irradiation may be more Simple, sensitive, safe and effective in vitro prevention of GVHD may be more suitable for CB transplantation.