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抵抗素(resistin)是小鼠白色脂肪组织大量表达的富含半胱氨酸的分泌型蛋白.近年研究发现,人与啮齿类动物的抵抗素组织表达分布存在很大差异.小鼠抵抗素主要在白色脂肪组织表达,而人抵抗素主要在单核细胞/巨噬细胞表达,且在骨髓组织中大量表达,但目前骨髓中的细胞定位还不清楚.本研究的目的是明确成人骨髓及外周血白细胞中抵抗素表达细胞的类型.免疫荧光法检测骨髓中抵抗素表达细胞,结果显示,抵抗素主要表达在细胞核呈杆状和分叶核状的成熟粒细胞中,其中杆状核粒细胞表达较高,分叶核粒细胞表达减弱.Anti-hresistin IgG-Biotin-PE单色荧光流式细胞术分选外周血白细胞中抵抗素表达细胞后经瑞氏化学染色,结果显示,抵抗素表达细胞主要为杆状和分叶核状的嗜中性粒细胞,还有少量嗜酸性粒细胞,且抵抗素蛋白分布在细胞质中.RT-qPCR结果在RNA水平上证明,人抵抗素在嗜中性粒细胞中大量表达.Anti-hresistin IgG-FITC和anti-HNL IgG-Biotin-PE双色荧光流式细胞术进一步证明,抵抗素的主要表达细胞为成熟的嗜中性粒细胞.嗜中性粒细胞在机体免疫防御中起重要作用,人骨髓及外周血中抵抗素主要在成熟嗜中性粒细胞中表达,这一研究结论为人抵抗素与炎症反应的关联性及其功能的进一步研究奠定了基础.
Resistin (resistin) is a cysteine-rich secretory protein that is abundantly expressed in mouse white adipose tissue .Recent studies have found that resistin distribution in humans and rodents varies widely. Is expressed in white adipose tissue, and human resistin is mainly expressed in monocytes / macrophages and is abundantly expressed in bone marrow, but the location of cells in the bone marrow is unclear at present. The purpose of this study was to identify the bone marrow and peripheral The type of resistin-expressing cells in the blood leucocytes.The expression of resistin in the bone marrow was detected by immunofluorescence.The results showed that the resistin was mainly expressed in the mature granulocytes with rod-like and lobulated nuclei in the nucleus, of which the granulocytes The expression of which was higher and the expression of mitochondria granule cells was weakened.Anti-hresistin IgG-Biotin-PE monochrome fluorescence flow cytometry was used to sort out the resistin-expressing cells in the peripheral blood leukocytes and then analyzed by Wright’s chemical staining. The results showed that the expression of resistin The cells mainly consisted of rod-shaped and lobulated nucleated neutrophils, a small amount of eosinophils and the resistin protein distributed in the cytoplasm.RT-qPCR results demonstrated at the RNA level, Resistin was abundantly expressed in neutrophils.Anti-hresistin IgG-FITC and anti-HNL IgG-Biotin-PE bi-color fluorescence flow cytometry further demonstrated that the major expressed resistin cells were mature neutrophils . Neutrophils play an important role in the body’s immune defense. The resistance of human bone marrow and peripheral blood is mainly expressed in mature neutrophils. The conclusion of this study is that the relationship between human resistin and inflammatory reaction and its function The further study laid the foundation.