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目的:探讨不同时程温和应激对大鼠海马神经元、外周血T淋巴细胞亚群的影响。方法:采用温和应激刺激建立不同应激时程动物模型,以血清皮质醇和旷场行为作为应激状态的指标,观察不同时程温和应激对大鼠海马神经元形态、外周血T淋巴细胞亚群的动态影响。结果:温和应激第7天,海马神经元肿胀、淡染、排列稀疏;应激第14天,细胞萎缩,间隙增大,细胞层次变稀、中断;应激第21天,出现大量细胞坏死。外周血CD3+、CD4+T淋巴细胞数在应激7天组、14天组和21天组显著低于对照组。CD4/CD8+比率在应激7天组、14天组显著低于对照组。T淋巴细胞亚群的变化趋势为CD3+、CD4+T淋巴细胞数和CD4/CD8+比率均在应激7天降低,应激14天时为最低水平,应激21天回升。旷场行为在应激7天、应激14天、应激21天均显著高于对照组,其中应激14天旷场运动得分最高。血清皮质醇水平在应激7天组和14天组显著高于对照组,应激21天时回落。结论:温和应激7天导致海马神经元损伤、细胞免疫功能抑制和稳态失衡,明显紊乱出现在应激14天,持续至应激21天。外周血T淋巴细胞亚群可作为应激的指标之一。
Objective: To investigate the effects of mild stress at different time courses on T lymphocyte subsets in hippocampal neurons and peripheral blood of rats. Methods: Different stress duration animal models were set up with gentle stress stimulation. Serum cortisol and open field behaviors were taken as the indicators of stress state. The effects of mild stress at different time courses on the morphology of hippocampal neurons, peripheral blood T lymphocytes Dynamic impact of subgroups. Results: On the 7th day of mild stress, hippocampal neurons became swollen, lightly stained and sparsely arranged. On the 14th day of stress, the cells atrophied, the gap increased, and the cell layer became thinner and interrupted. On the 21st day of stress, a large amount of cell necrosis . The number of CD3 + and CD4 + T lymphocytes in peripheral blood was significantly lower in 7-day stress group, 14-day group and 21-day stress group than in control group. CD4 / CD8 + ratio in stress 7 days group, 14 days group was significantly lower than the control group. The trends of T lymphocyte subsets were CD3 +, CD4 + T lymphocyte count and CD4 / CD8 + ratio. The T lymphocyte subsets decreased at 7 days and the lowest at 14 days. Open-field behavior in stress 7 days, stress 14 days, stress 21 days were significantly higher than the control group, including stress 14 days open field exercise highest score. Serum cortisol levels in stress 7 days and 14 days group was significantly higher than the control group, the stress 21 days down. CONCLUSION: Gentle stress induces neuronal damage in hippocampus, inhibition of cellular immunity and homeostasis in 7 days of stress. The apparent disturbance occurs in 14 days of stress and lasts for 21 days. Peripheral blood T lymphocyte subsets can be used as an indicator of stress.