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目的观察沙利度胺和三氧化二砷(As2O3)对急性非淋巴细胞白血病(ANLL)细胞分泌血管内皮生长因子(VEGF)的影响,并观察二者是否有协同性。方法对2001-10~2003-02中国医科大学第二临床学院的23例ANLL病人的骨髓,用Ficoll淋巴细胞分离液分离单个核细胞(MNC),培养于RPMI1640培养液中,于37℃、5%CO2饱和湿度条件下传代培养。以2×109/L的细胞加入24孔培养板。分为空白对照组、沙利度胺组(300μg·mL-1)、As2O3组(25μmol/L)和联合用药组,连续培养72h。用ELISA法检测上清液VEGF质量浓度。结果4组VEGF质量浓度分别为(1582·67±281·51)ng/L、(1206·29±264·87)ng/L、(941·72±236·34)ng/L和(639·12±211·98)ng/L。用药组与空白对照组及联合用药组与单一用药组之间差异均有显著性(P<0·05)。结论(1)白血病细胞能自分泌VEGF。(2)沙利度胺和As2O3均能抑制ANLL细胞分泌VEGF。(3)两者联合应用起协同作用。
Objective To observe the effects of thalidomide and As2O3 on the secretion of vascular endothelial growth factor (VEGF) in acute non-lymphocytic leukemia (ANLL) cells and observe whether they have synergism. Methods Twenty-three patients with ANLL from the Second Clinical College of China Medical University from October 2001 to February 2003 were enrolled in this study. Mononuclear cells (MNCs) were isolated by Ficoll lymphocyte separation and cultured in RPMI1640 medium at 37 ℃ for 5 % CO2 saturated humidity subculture. The cells were added to 24-well plates at 2 x 109 / L. The cells were divided into three groups: control group, thalidomide group (300μg · mL-1), As2O3 group (25μmol / L) and combination group. Supernatant VEGF concentration was detected by ELISA. Results The VEGF concentrations in the four groups were (1582.67 ± 281.51) ng / L, (1206.29 ± 264.77) ng / L, (941.72 ± 236.34) ng / L and 12 ± 211 · 98) ng / L. There was significant difference between medication group, blank control group, combination therapy group and single treatment group (P <0.05). Conclusions (1) Leukemia cells can autocrine VEGF. (2) Both thalidomide and As2O3 could inhibit the secretion of VEGF in ANLL cells. (3) the combination of the two play a synergistic effect.