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目的探讨趋化因子受体(chemokine receptors,CRs)、基质金属蛋白酶(matrix metalloproteinases,MMPs)、血管内皮生长因子(vascular endothelial growth factor,VEGF)的表达与胰腺癌肿瘤相关成纤维细胞(cancer associated fibroblasts,CAFs)生物学行为的关系。方法用原代培养的方法建立胰腺癌CAFs和正常成纤维细胞(normal fibroblasts,NFs)。用Real-time PCR方法检测CRs(CXCR1、CXCR2、CXCR4、CXCR7)、MMPs(MMP1、MMP2、MMP7、MMP9)和VEGF m RNA的表达。结果成功建立胰腺癌CAFs和正常胰腺NFs。在CAFs和NFs中,趋化因子受体CXCR1、CXCR2、CXCR4、CXCR7 m RNA的表达均存在差异,CAFs表达量高于NFs。其中CXCR2、CXCR4 m RNA在两株细胞中的表达差异具有统计学意义(P=0.010,P=0.049)。MMPs和VEGF m RNA的表达同样存在差异,CAFs中MMP7和VEGF m RNA表达明显高于NFs,差异具有统计学意义(P=0.017,P=0.049);CAFs中MMP9 m RNA的表达明显低于NFs,差异具有统计学意义(P=0.012)。结论在胰腺癌CAFs中CRs、MMPs和VEGF m RNA的表达有差异,可能与其促侵袭转移能力存在一定的相关性。
Objective To investigate the expression of chemokine receptors (CRs), matrix metalloproteinases (MMPs), vascular endothelial growth factor (VEGF) and pancreatic cancer associated fibroblasts , CAFs) biological behavior. Methods Primary culture was used to establish pancreatic cancer CAFs and normal fibroblasts (NFs). The expression of CRs (CXCR1, CXCR2, CXCR4, CXCR7), MMPs (MMP1, MMP2, MMP7, MMP9) and VEGF m RNA were detected by Real-time PCR. Results Successful establishment of pancreatic cancer CAFs and normal pancreatic NFs. The expression of CXCR1, CXCR2, CXCR4 and CXCR7 m RNA in CAFs and NFs were all different, and the expression of CAFs was higher than NFs. Among them, the expression of CXCR2 and CXCR4 m RNA in the two cell lines was significantly different (P = 0.010, P = 0.049). The expression of MMPs and VEGF m RNA also differed in CAFs, and the expression of MMP7 and VEGF m RNA in CAFs was significantly higher than NFs (P = 0.017, P = 0.049). The expression of MMP9 m RNA in CAFs was significantly lower than that in NFs , The difference was statistically significant (P = 0.012). Conclusions The expression of CRs, MMPs and VEGF m RNA in CAFs of pancreatic cancer is different, which may be related to their ability of promoting invasion and metastasis.