论文部分内容阅读
目的:研究甲基莲心碱对人肝癌HepG2细胞增殖及凋亡的影响。方法:采用CCK-8法观察甲基莲心碱对人肝癌细胞HepG2细胞生长增殖的影响;Hoechst33258染色观察甲基莲心碱作用HepG2细胞后细胞形态的变化;测定乳酸脱氢酶(LDH)释放率观察HepG2细胞受损程度;Annexin V-PI双染测定甲基莲心碱对HepG2细胞凋亡率的影响;PI/RNase单染检测不同浓度的甲基莲心碱对其凋亡周期的影响。结果:甲基莲心碱对体外培养的人肝癌HepG2细胞的生长具有抑制作用,且呈剂量、时间依赖性;细胞膜的受损程度随着药物剂量的增大逐步提高;Hoechst33258及流式结果表示,甲基莲心碱可使HepG2细胞阻滞于G_0/G_1期,细胞凋亡率随药物浓度的增大亦呈增长趋势。结论:甲基莲心碱可显著抑制肝癌HepG2细胞的生长增殖,并在一定的范围内呈剂量、时间依赖性,诱导其阻滞于G_0/G_1期,发生晚期凋亡。
Objective: To study the effect of neferine on the proliferation and apoptosis of human hepatoma HepG2 cells. Methods: The effect of neferine on the growth and proliferation of HepG2 cells was observed by CCK-8 method. The morphological changes of HepG2 cells were observed by Hoechst33258 staining. The release rate of lactate dehydrogenase (LDH) was measured HepG2 cells were harvested. Annexin V-PI double staining was used to determine the effect of neferine on the apoptotic rate of HepG2 cells. PI / RNase staining was used to detect the effect of different concentrations of neferine on the apoptotic cycle. Results: Neferine could inhibit the growth of human HepG2 cells in vitro in a dose-and time-dependent manner. The damage of cell membrane was gradually increased with the increase of the dose of Hoechst 33258 and the flow cytometry results. Neferine blocked HepG2 cells in G_0 / G_1 phase, and apoptosis rate increased with the increase of drug concentration. Conclusion: Neferine can significantly inhibit the growth and proliferation of HepG2 cells in a dose - and time - dependent manner and induce its arrest in G_0 / G_1 phase with advanced apoptosis.