论文部分内容阅读
在水稻品种南粳41中发现了一个黄绿叶自然突变体,经过多代连续自交形成了稳定的突变系,命名为ygl11(t),ygl11(t)整个生育期叶片都表现为黄绿色。对苗期、分蘖盛期、齐穗期突变体和野生型的叶绿素含量进行测定,ygl11(t)的叶绿素含量是野生型的45.7%~74.7%,叶绿素a含量是野生型的55.2%~87.5%,叶绿素b含量是野生型的12.5%~25.3%,ygl11(t)的类胡萝卜素的含量是野生型的62.3%~97.0%。ygl11(t)在分蘖盛期的净光合速率显著高于野生型,花后10d,ygl11(t)的净光合速率比野生型略低。对突变体叶片中叶绿体的超微结构进行观察,发现突变体叶绿体内的类囊体基粒片层数目减少且严重扭曲变形。遗传分析表明,ygl11(t)叶色性状受1对隐性核基因控制。利用SSR分子标记将YGL11(t)初步定位在水稻第10染色体的长臂上,进一步利用新开发的InDel和CAPS标记将YGL11(t)定位在58.1kb的物理距离内。对该区段内存在的开放阅读框进行序列分析,发现突变体ygl11(t)中编码叶绿素a氧化酶(chlorophyll a oxygenase 1)基因(OsCAO 1)的第9个外显子存在2个碱基缺失,从而导致提前出现终止密码子,初步分析OsCAO1即为YGL11(t)的候选基因。
A yellow-green leaf natural mutant was found in the rice variety Nanjing41, and a stable mutant line was formed after many generations of self-fertilization. The mutant was named ygl11 (t), ygl11 (t). The content of chlorophyll of ygl11 (t) was 45.7% ~ 74.7% of the wild type and the content of chlorophyll a was 55.2% ~ 87.5% of the wild type at seedling stage, tillering stage, heading stage and wild type. %, Chlorophyll b content is 12.5% ~ 25.3% of the wild type, ygl11 (t) carotenoid content is 62.3% ~ 97.0% of the wild type. The net photosynthetic rate of ygl11 (t) at tillering stage was significantly higher than that of wild type. The net photosynthetic rate of ygl11 (t) was slightly lower than that of wild type at 10 days after anthesis. The ultrastructure of chloroplasts in mutant leaves was observed and found that the number of thylakoid lamellae in the mutant chloroplast decreased and severely distorted. Genetic analysis showed that leaf color traits of ygl11 (t) were controlled by one pair of recessive nuclear genes. YGL11 (t) was initially located on the long arm of rice chromosome 10 using SSR molecular markers. YGL11 (t) was further mapped to a physical distance of 58.1 kb using newly developed InDel and CAPS markers. Sequence analysis of the open reading frame in this region revealed that there was 2 bases in the 9th exon of the gene encoding the chlorophyll a oxygenase 1 gene (OsCAO 1) in ygl11 (t) Deletion, resulting in premature stop codon, preliminary analysis of OsCAO1 YGL11 (t) candidate genes.