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目的:优化CCK-8试剂盒在血液毒理学检测中的应用条件。方法:以CCK-8试剂盒标准检测法和两种优化的方法分别检测不同浓度的氢醌(hydroquinone,HQ)对TK6细胞增殖速度的影响,分别与计数法比较,分析影响实验检测的原因。结果:试剂盒标准方法中,HQ对CCK-8试剂的检测结果有影响,结果出现偏差,优化后的两种方法与计数法检测结果较为相似。结论:HQ对CCK-8试剂检测结果有影响,优化出一种适用于HQ血液毒理学检测的细胞增殖方法。
Objective: To optimize the application conditions of CCK-8 kit in hematology and toxicology. Methods: The effects of different concentrations of hydroquinone (HQ) on the proliferation rate of TK6 cells were detected by CCK-8 kit standard assay and two optimized methods, respectively, compared with the counting method to analyze the reasons that affect the experimental test. Results: In the standard method of kit, HQ had influence on the detection results of CCK-8 reagent, and the results showed deviation. The optimized two methods were similar to the counting method. Conclusion: HQ has an influence on the detection results of CCK-8 reagent, and a cell proliferation method suitable for HQ hematology toxicology test is optimized.