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AIM To demonstrate the relationship betweenH-ras oncogene and hepatocellular carcinoma(HCC)metastasis.METHODS Activated H-ras oncogene wastransfected into SMMC 7721,a cell line derivedfrom human HCC,by calcium phosphatetransfection method.Some metastasis-relatedparameters were detected in vitro,includingadhesion assay,migration assay,expression ofcollagenase Ⅳ(c Ⅳ ase)and epidermal growthfactor receptor(EGFR).RESULTS The abilities of H-ras-transfected cellclones in adhesion to laminin(LN)or fibronectin(FN),migration,c Ⅳ ase secretion increasedmarkedly,and the expression of EGFR elevatedmoderately.More importantly,these alterationswere consistent positively with the expressionof p21,the protein product of H-ras oncogene.CONCLUSION H-ras oncogene could inducethe metastatic phenotype of HCC cell in vitro toraise its metastatic potential.
AIM To demonstrate the relationship between H-ras oncogene and hepatocellular carcinoma(HCC)metastasis.METHODS Activated H-ras oncogene wastransfected into SMMC 7721,a cell line derivedfrom human HCC,by calcium phosphatetransfection method.Some metastasis-relatedparameters were detected in vitro,includingadhesion Assay,migration assay,expression of collagenase IV(c IV ase) and epidermal growth factor receptor (EGFR).RESULTS The abilities of H-ras-transfected cell clones in adhesion to laminin(LN) or fibronectin(FN),migration,c IV ase secretion Increasedmarkedly,and the expression of EGFR elevatedmoderately.More importantly,the alterationswere consistently positively with the expressionof p21,the protein product of H-ras oncogene.CONCLUSION H-ras oncogene could inducethe metastatic phenotype of HCC cell in vitro toraise its metastatic potential.