黑色素瘤是一种常见的恶性肿瘤,随着恶性程度的增高,黏着斑激酶(FAK)水平递增。本文利用RNAi技术,成功建立了稳定干扰FAK基因的F10-siFAK及阴性对照F10-control两种细胞系。与对照F10-control相比,F10-siFAK细胞系mRNA表达下降,蛋白表达减少,ERK磷酸化显著降低,细胞周期在G1期阻滞增加,而且在动物体内的生长速度明显降低。稳定干扰FAK基因的黑色素瘤细胞株的建立,为研究FAK在黑色素瘤进程中的作用提供了工具,并为以FAK为靶点的黑色素瘤治疗药物的筛选提供了平台。 Melanoma is a common malignant tumor with increasing levels of focal adhesion kinase (FAK) as the degree of malignancy increases. In this paper, we successfully established F10-siFAK and F10-control cell lines that stably interfere with FAK gene by using RNAi technique. Compared with the control F10-control, F10-siFAK cell line mRNA expression decreased, protein expression decreased, ERK phosphorylation was significantly reduced, cell cycle arrest in G1 phase increased, and the growth rate was significantly reduced in animals. The establishment of melanoma cell line stably interfering with FAK gene provides a tool to study the role of FAK in melanoma progression and provides a platform for the screening of FAK-targeted melanoma therapeutic agents.