以多寄主可移动载体质粒pSUP1011为基础载体，构建了绿色荧光蛋白（GFP）标记的重级质粒，通过细菌接合，将重组质粒转移到嗜水气单胞菌J－l中。经紫外光检测和SDS－PAGE电泳分析，证实重组质粒在该富中可表达GFP。在无选择压力的培养条件下，24h后，重组质粒的稳定率在72．2％；36h后，稳定率在50％。显示GFP作为报告基因在嗜水气单胞菌致病机理研究的应用前景。 The recombinant plasmids were transferred into Aeromonas hydrophila J-1 by bacterial conjugation using the multi-host mobile vector pSUP1011 as the basic vector to construct a green fluorescent protein (GFP) -tagged heavy plasmid. After UV detection and SDS-PAGE electrophoresis analysis, it was confirmed that the recombinant plasmid can express GFP in the rich. Under the condition of no selective pressure, the stability of the recombinant plasmid was 72.2% after 24 h, and the stability was 50% after 36 h. The application of GFP as a reporter gene in the pathogenesis of Aeromonas hydrophila has been shown.