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为研究小鼠胎盘细胞同种异体移植的抗衰老作用,将15月龄雌性BALB/c老年小鼠分为对照组(A)、短期移植组(B)以及长期多次移植组(C)共3组;取孕18 d小鼠胎盘,50目细胞筛网研磨胎盘,制备小鼠胎盘细胞悬液(1×107cells/ml),经腹腔注入B组和C组小鼠体内,1 ml/只,每10 d注射1次,连续注射3次。之后,再继续对C组小鼠不定期多次注射胎盘细胞悬液,直至实验结束。移植3个月后评价B组小鼠心脏结构和功能的各指标,心脏质量指数(心脏质量/体质量)和脾脏质量指数(脾脏质量/体质量),心脏、肾脏、肺脏、皮肤等器官组织学衰老程度分值,血清总超氧化物歧化酶活力、丙二醛含量以及谷胱甘肽过氧化物酶活力,皆优于A组(均P<0.05);且B组小鼠存活日期为A组的1.7倍,而B、C组之间无统计学差异;同时,Y染色体原位杂交实验表明,B组和C组小鼠多个脏器内均可检测到长期存活的移植细胞。结果提示:移植小鼠胎盘细胞可在一定程度上延缓小鼠衰老进程。
To investigate the anti-aging effects of placental cell allograft in mice, 15-month old female BALB / c mice were divided into control group (A), short-term transplantation group (B) and long-term multiple transplantation group The placenta of placenta of mice were inoculated on the 18th day of pregnancy and the placenta of 50 cells were grinded. The placental cell suspension (1 × 107cells / ml) was prepared and injected into the mice of group B and group C , Every 10 d injection 1, continuous injection 3 times. After that, continue to group C mice from time to time multiple placental cell suspension injection, until the end of the experiment. Three months after transplantation, the indexes of heart structure and function, heart mass index (heart mass / body mass), spleen mass index (spleen mass / body weight), heart, kidney, lungs, skin and other organs and tissues Aging scores, serum total superoxide dismutase activity, malondialdehyde content and glutathione peroxidase activity were better than those in group A (all P <0.05); and the survival time of group B was A group 1.7 times, but no significant difference between B, C group; the same time, Y chromosome in situ hybridization experiments showed that B and C mice can be detected in multiple organs long-term survival of transplanted cells. The results suggest that transplanted mouse placental cells can delay the aging process of mice to a certain extent.