【摘 要】
：
The anther cuticle and pollen exine play a critical role in male gametophyte development. The sporopol-lenin precursors and cuticular lipid monomers are transported to the surface of the microspores and the epidermis by lipid transport proteins (LTPs) and
【机 构】
：
Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081,China
论文部分内容阅读
The anther cuticle and pollen exine play a critical role in male gametophyte development. The sporopol-lenin precursors and cuticular lipid monomers are transported to the surface of the microspores and the epidermis by lipid transport proteins (LTPs) and ATP-binding cassette G (ABCG) transporters for the for-mation of the pollen wall and anther cuticle, respectively. However, the function of ABCG transporters in maize anther development is unclear. Here, we cloned the MS2 gene from the maize male sterile2 mutant using map-based cloning and determined that it encodes an ABCG transporter. MS2 protein was exper-imentally confirmed to be located on the cell membrane. The quantitative real-time PCR (qRT-PCR) results showed that MS2 was ubiquitously expressed in all vegetative and reproductive tissues, whereas a high transcriptional level of MS2 was observed in anthers, especially at the young microspore stage. Gas chromatography-mass spectrometry (GC-MS) analysis showed decreased accumulation of cutin and wax components in ms2 anthers, indicating that MS2 plays a role in the transport of lipid molecules to anther cuticle and pollen exine. To our knowledge, MS2 is the first reported ABCG transporter gene that partic-ipates in anther development in maize.
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