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目的了解河北省HIV-1亚型的分布特点和传播方式,推测流行时间,预测流行趋势。方法采集HIV感染者的全血样品,分离外周血单核细胞(PBMC),提取前病毒DNA,使用套式聚合酶链反应(nested-PCR),扩增HIV-1的env基因的C2-V3区并进行序列测定和亚型分析。结果对22份HIV-1感染者的样品,扩增得到了18份HIV-1envC2-V3基因片段,经序列测定和基因分析鉴定出3种HIV-1M亚群基因亚型,即:B′、CRF-BC和C亚型。B′亚型的组内基因离散率为7.84%±3.14%(n=14),基因序列与云南瑞丽株rl42(泰国B亚型)相近;2株CRF-BC亚型与广西毒株的基因离散率为4.60%。与血液途径感染有关的人员均为B′(泰国B)亚型。结论目前,在河北省发现了3种HIV-1亚型。输供血途径中B′亚型仍是主要的流行亚型,可能来源于云南吸毒人群。HIV-1B′亚型在河北省的流行时间大约为7~9年。
Objective To understand the distribution and transmission of HIV-1 subtype in Hebei Province, predict the prevalence of HIV-1 and predict the epidemic trend. Methods Whole blood samples of HIV-infected individuals were collected. Peripheral blood mononuclear cells (PBMCs) were isolated and the proviral DNA was extracted. Using nested-PCR, C2-V3 of env gene of HIV-1 Regions were sequenced and subtype analyzed. RESULTS: Twenty-eight HIV-1 envC2-V3 gene fragments were amplified from 22 samples of HIV-1 infected individuals. Three subtypes of HIV-1 subgroups were identified by sequence analysis and gene analysis: B ’ CRF-BC and C subtypes. The genotype frequency of B subtypes was 7.84% ± 3.14% (n = 14), and the gene sequence was similar to Yunnan Ruili strain rl42 (Thai subtype B). The genotypes of two CRF-BC subtypes and Guangxi strains The dispersion rate is 4.60%. Persons involved in bloodstream infections are B ’(Thai B) subtypes. Conclusions At present, three HIV-1 subtypes have been found in Hebei Province. The B subtypes in the supply and delivery route are still the major epidemic subtypes, probably originating from Yunnan drug users. The prevalence of HIV-1B subtype in Hebei province is about 7-9 years.