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为了探讨运动性闭经及月经失调的发病机制，选择了５３名女运动员作为研究对象。采用放免法（ＲＩＡ）测定闭经组、长月经周期组和对照组血浆基础β—内啡肽（β－ＥＰ）、促性腺激素释放激素（ＧｎＲＨ）、黄体生成素（ＬＨ）、卵泡刺激素（ＦＳＨ）的浓度。结果表明闭经组和长周期组的ＧｎＲＨ值显著低于对照组（Ｐ＜０．０５）。对闭经组进行垂体兴奋试验表明，其垂体未发生器质性病变。用ＲＩＡ法测定长月经周期组、短月经周期组和对照组唾液基础雌二醇（Ｅ_２）、孕酮（Ｐ）浓度。结果表明，长、短周期组Ｅ_２和Ｐ浓度均低于对照组（Ｐ＜０．０５）。
In order to explore the pathogenesis of exercise-induced amenorrhea and menstrual disorders, 53 female athletes were selected as research subjects. The levels of plasma β-EP, GnRH, LH, FSH (α1) in the amenorrhea group, long menstrual cycle group and control group were determined by radioimmunoassay (RIA) FSH) concentration. The results showed that the GnRH values in the amenorrhea group and the long-term group were significantly lower than those in the control group (P <0.05). Pituitary excitatory test on amenorrhea group showed that there was no organic lesion in the pituitary. The concentrations of salivary estradiol (E2) and progesterone (P) in long menstrual cycle group, short menstrual cycle group and control group were determined by RIA. The results showed that the concentrations of E_2 and P in long-term and short-term groups were lower than those in control group (P <0.05).