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为了探明猪粪堆肥升温期的细菌多样性和堆肥过程中由“梯度效应”导致的细菌空间分布差异,采集了堆体上、中、下3层的堆肥样品,对其进行了16S rDNA序列和PCR-DGGE分析。序列分析结果表明:从3层样品中获得的121条16S rDNA序列,其中的113条与GenBank数据库中已知菌的16S rDNA序列表现出了同源性,且大多数与梭菌属有较近的亲缘关系,7条序列与已知菌的序列无任何相似性。上层样品中Shigellasp.、Acinetobactersp.和Clostridiumsp.占优势;中层样品中Clostridiumsp.占绝对优势;下层样品中Clostridiumsp.和Lactobacillussp.占优势。PCR-DGGE结果显示:3层堆肥样品都具有较丰富的微生物种群多样性,同时存在着明显的优势种群结构变化,中层多样性最为丰富。
In order to find out the bacterial diversity in pig manure composting and the difference in bacterial distribution caused by the “gradient effect” in compost process, compost samples of upper, middle and lower three layers were collected and subjected to 16S rDNA sequence and PCR-DGGE analysis. Sequence analysis revealed that 113 of the 121 16S rDNA sequences obtained from the three-layer samples showed homology with the 16S rDNA sequences of known bacteria in the GenBank database, and most of them were close to Clostridium There are no similarities between the seven sequences and those of known bacteria. Shigellasp., Acinetobactersp. And Clostridiumsp. Are dominant in the upper samples; Clostridium sp. Is dominant in the middle samples; Clostridium sp. And Lactobacillussp. Are dominant in the lower samples. PCR-DGGE results showed that all three layers of compost samples had rich diversity of microbial population, meanwhile there were obvious structural changes of dominant population with the middle layer of diversity being the most abundant.