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目的:观察隔药灸命门穴治疗对痛经模型大鼠子宫内膜的孕激素受体(PR)、雌激素受体(ER)表达量的作用机制。方法:将雌性SD大鼠随机分为空白组(A组)和造模组,构建原发性痛经的老鼠模型,造模成功后,将痛经老鼠模型随机分为模型组(B组)、药物组(C组)、针刺组(D组)、隔药灸组(E组)。观察各组大鼠出现扭体反应的潜伏期、30min内扭体次数及大鼠子宫内膜PR、ER表达水平。结果:模型组发生扭体反应次数较多,且扭体反应前有潜伏期,空白组无扭体反应(P<0.01)。针刺组、药物组、隔药灸组大鼠扭体反应次数均较模型组减少,且扭体潜伏期均延长(P<0.01)。隔药灸组和针刺组大鼠扭体反应次数显著降低(P<0.01);隔药灸组大鼠扭体反应较针刺组少(P<0.01)。隔药灸组大鼠扭体抑制率最高(P<0.05)。隔药灸组、针刺组、药物组及模型组大鼠ER表达较空白组显著增加,PR表达显著减少(P<0.01);隔药灸组、针刺组及药物组大鼠ER表达较模型组显著减少,PR表达显著增加(P<0.01);针刺组及隔药灸组大鼠ER表达较药物组显著降低,PR表达显著增加(P<0.01);隔药灸组大鼠ER表达较针刺组显著减少(P<0.01),PR表达显著增加(P<0.01)。结论:隔药灸治疗原发性痛经与调节ER、PR表达有关,可下调痛经大鼠ER表达,提高PR表达。
OBJECTIVE: To observe the mechanism of action of moxibustion at Momenguone on the expression of progesterone receptor (PR) and estrogen receptor (ER) in endometrium of dysmenorrheal model rats. Methods: Female Sprague-Dawley rats were randomly divided into blank group (group A) and model group to construct a mouse model of primary dysmenorrhea. After the model was successfully established, the model of dysmenorrhea rats were randomly divided into model group (group B), drug Group C, acupuncture group D and drug-partition moxibustion group E. The latency of writhing reaction, the number of writhing in 30 min and the expression of PR and ER in rat endometrium were observed. Results: There were many writhing reactions in the model group, and there was an incubation period before the writhing reaction. There was no writhing reaction in the blank group (P <0.01). The numbers of writhing reaction in acupuncture group, drug group and moxibustion group were all decreased compared with those in model group, and the writhing latency was prolonged (P <0.01). The numbers of writhing reaction in moxibustion group and acupuncture group were significantly lower than those in acupuncture group (P <0.01). The moxibustion group had the highest writhing rate (P <0.05). Compared with the blank group, the ER expression of the moxibustion group, the acupuncture group, the drug group and the model group increased significantly and the expression of PR decreased significantly (P <0.01). Compared with the moxibustion group, acupuncture group and the drug group, (P <0.01). The expression of ER in acupuncture group and moxibustion moxibustion group was significantly lower than that in drug group and PR expression was significantly increased (P <0.01) Compared with acupuncture group, the expression of PR was significantly decreased (P <0.01), PR expression was significantly increased (P <0.01). Conclusion: Moxibustion moxibustion treatment of primary dysmenorrhea and regulation of ER, PR expression, dysmenorrhea rats ER expression can be reduced to improve PR expression.