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目的利用大鼠肠内厌氧细菌对虎杖中主要单体虎杖苷进行生物修饰研究,观察虎杖苷在大鼠体内及体外经肠内菌代谢后的变化并确定代谢物。方法生物样品经处理后分别进行LC-MS/MS及HPLC检测分析。结果从虎杖苷的大鼠肠内厌氧细菌培育物中检出2个化合物,即虎杖苷及大鼠肠内厌氧细菌代谢产物白藜芦醇;大鼠ig虎杖苷1 500 mg.kg-1后提取4 h胃肠道内容物,证明肠道存在虎杖苷和代谢产物白藜芦醇。结论虎杖苷可被大鼠肠内菌代谢,其代谢模式为虎杖苷→白藜芦醇。
OBJECTIVE: To study the biological modification of polydatin, a major monomer in Polygonum cuspidatum, by anaerobic bacteria in the intestine of rats, and to study the changes of the polydatin metabolism in vivo and in vitro after the metabolism of polydatin in rats. Methods The biological samples were analyzed by LC-MS / MS and HPLC respectively. Results Two compounds were detected from the intestinal anaerobic bacterial cultures of polydatin, ie, polydatin and rat intestinal anaerobic bacterial metabolites resveratrol; rat ig polydatin 1500 mg.kg- 1 4h after extraction of the contents of the gastrointestinal tract to prove that the presence of cedar pollen glycosides and metabolites of resveratrol. Conclusion Polydatin can be metabolized by intestinal bacteria in rats. The metabolic model is polydatin → resveratrol.