论文部分内容阅读
背景 流体静力学-压力病毒灭活技术是一项新的灭活血浆和血液成分中病原体的方法,它能保持这些制品的疗效。研究设计和方法 使用一特制的设备对人血浆样品加压以杀死λ噬菌体。在加压处理后测定噬菌体的滴度和血浆蛋白的活性。结果 研究发现压力灭活λ噬菌体是一个有效的病毒灭活方法,尤其是在0℃左右效果优于20℃以上及-40℃以下的效果。提高压力和常压与高压循环交替进行可提高灭活效率。但血浆蛋白碱性磷酸酯酶和总淀粉酶的活性未随温度而变化,且在相同压力处理后,分别保持在开始时的29％和6％以内。结合常压与高压循环、接近0℃、高压的条件下处理20分钟后,血浆中
Background Hydrostatics - A stress-virus inactivation technique is a new method of inactivating pathogens in plasma and blood components that maintains the efficacy of these preparations. Study Design and Methods A special device was used to pressurize human plasma samples to kill lambda phage. The phage titer and the plasma protein activity were measured after the pressurization. Results It was found that pressure inactivation of λ phage was an effective virus inactivation method, especially at 0 ° C and better than above 20 ° C and below -40 ° C. Increasing pressure and alternating atmospheric and high pressure cycles can increase inactivation efficiency. However, plasma protein alkaline phosphatase and total amylase activity did not change with temperature and remained within 29% and 6%, respectively, of the beginning after the same pressure treatment. Combined with atmospheric and high pressure cycles, close to 0 ℃, high pressure treatment for 20 minutes, the plasma