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为解决香蕉愈伤组织诱导培养过程中的外植体的褐化现象,分别用AgNO3(10,20 mg·L-1),AC(1,2,3 g·L-1),PVP(1,2 g·L-1),VitC(100,200 mg·L-1)等防褐化剂,对香蕉的愈伤组织诱导进行防褐化研究。结果表明:外植体培养10 d后,所有防褐化处理实验组的褐化率均低于对照组,说明在愈伤组织诱导的早期,不同的防褐化剂均有一定的防褐化效果;随着培养时间延长,不同的防褐化处理逐渐出现差异,在愈伤组织诱导培养40 d后,活性炭的防褐化效果最好,其中1 g·L-1活性炭的褐化率仅为20%,效果最佳。
In order to solve the browning phenomenon of explants during the induction culture of banana callus, the effects of AgNO3 (10, 20 mg · L-1), AC (1,2,3 g · L -1), PVP , 2 g · L-1) and VitC (100, 200 mg · L-1) were used to study the callus induction of banana. The results showed that after the explants were cultured for 10 days, the browning rates of all browning treatment groups were lower than that of the control group, indicating that in the early stage of callus induction, different browning inhibitors have some browning resistance The results showed that the anti-browning treatment gradually became different with the prolongation of culture time. After 40 days of callus induction, the anti-browning effect of activated carbon was the best, and the browning rate of 1 g · L-1 activated carbon was only 20%, the best effect.