利用核酶（Ribozyme，Rib．）基因转化番木瓜（CaricapapayaL．）探索培育抗番木瓜环斑病毒（PapayaRingspotVirus，PRV）品种的新途径。用三亲交配法将切割PRVRNA的Rib．基因的表达载体（P35s／NPTⅡ，Rib．）转入农杆菌LBA4404，采用农杆菌介导转化将Rib．基因和NPTⅡ基因导入番木瓜细胞的核基因组中。其培养的外植体在含有Kan．100μg／mL，Carb．500μg／mL的选择培养基上产生抗性的愈伤组织，并通过体胚途径再生植株。经DNAdotblot，Southernblot分子杂交检测证明，Rib．基因已整合到番木瓜再生植株的核基因组中。RNAdotblot分析和攻毒试验结果表明，核酶基因在转基因植株中获得了表达，转基因番木瓜植株对PRV具有一定的抗性。 A new pathway of cultivating anti-papaya ring spot virus (PRV) was explored by using Ribozyme (Rib.) Gene into Carica papaya L. (Carpapaya L.). The PRV RNA Ribs will be Cleaved by the Triple Parenting. The gene expression vector (P35s / NPTII, Rib.) Was transformed into Agrobacterium tumefaciens LBA4404, Agrobacterium-mediated transformation of Rib. The gene and NPTII gene are introduced into papaya cell nuclear genome. The cultured explants contained Kan. 100 μg / mL, Carb. Resistant callus was generated on 500 [mu] g / mL selection medium and plants were regenerated by the somatic embryo pathway. By DNAdotblot, Southern blot analysis of molecular hybridization proved Rib. The gene has been integrated into the nuclear genome of papaya regenerated plants. The result of RNAdotblot analysis and challenge experiment showed that the ribozyme gene was expressed in the transgenic plants and the transgenic plants had certain resistance to PRV.