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目的:研究新扶正除疫颗粒的血清药物化学。方法:通过SD大鼠灌胃给予新扶正除疫颗粒后,采用高效液相色清-二极管阵列检测器(HPLC-DAD)测定其含药血样色谱图,通过与空白样品的HPLC指纹图谱对比分析,探讨新扶正除疫颗粒的体内移行成分。结果:新扶正除疫血清HPLC色谱图中可检出14个移行成分,其中9个为原型成分入血,5个为代谢产物,通过对照品比对法(比较保留时间),确认了9个原型成分中4个成分分别为红景天苷、酪醇、虎杖苷、白藜芦醇。结论:含药血清的色谱峰明显多于空白样品,该方法为阐明新扶正除疫颗粒的药效物质基础提供科学依据。
Objective: To study the serum pharmacochemistry of Xinfuzheng Granules. Methods: SD rats were intragastrically given Xinfuzheng tablets, HPLC-DAD was used to determine the chromatogram of the drug-containing blood samples, and compared with the HPLC fingerprint of blank samples , To explore the new Fuzheng removal of particles in vivo migration components. Results: In the HPLC chromatogram of Xifuzheng serum, 14 migratory components were detected, of which 9 were blood samples of the prototype and 5 were metabolites. Nine samples were identified by the comparison method (comparison of retention time) The four components of the prototype components were salidroside, tyrosol, polydatin, resveratrol. Conclusion: There are more chromatographic peaks in drug-containing serum than blank samples. This method provides a scientific basis for elucidating the pharmacodynamic basis of Xinfuzheng Granules.