论文部分内容阅读
气孔是植物特化的表皮结构,在植物蒸腾过程和与外界气体交换过程中起到重要作用。拟南芥YDA(AtYDA)是MAPK级联信号途径中的一种激酶(MAPKKK4),它在叶片气孔的发育过程中起着负调控的作用。AtYDA功能缺失导致叶片气孔显著增加,而表达组成型激活形式的AtYDA(ΔN-YDA)则会导致表皮产生无气孔表型。本研究克隆了水稻中与AtYDA同源的2个基因OsYDA1和OsYDA2。在拟南芥中过量表达这2个基因都导致了叶片气孔密度的减少和叶片失水速率的降低。而表达ΔN-OsYDA1和ΔN-OsYDA2的转基因植株则呈气孔系数下降的表型。这表明OsYDA与AtYDA在调控气孔发育的功能上具有保守性。
Stomata are plant-specific epidermal structures that play an important role in the transpiration of plants and in the exchange of gases with the outside world. Arabidopsis YDA (AtYDA) is a MAPKKK4 kinase in the MAPK cascade signaling pathway and plays a negative regulatory role in leaf stomatal development. AtYDA loss resulted in a significant increase in leaf stomatal, while expression of constitutively active form of AtYDA (ΔN-YDA) resulted in a stomatal phenotype in the epidermis. In this study, we cloned two OsYDA1 and OsYDA2 genes homologous to AtYDA in rice. Overexpression of both genes in Arabidopsis resulted in a reduction of leaf stomatal density and a decrease of leaf water loss rate. Transgenic plants expressing ΔN-OsYDA1 and ΔN-OsYDA2 showed a phenotype with decreased stomatal coefficient. This indicates that OsYDA and AtYDA are conserved in their function of regulating stomatal development.