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目的 :探讨丙型肝炎病毒 (HCV)及恶性疟原虫 (Pf)复合DNA疫苗的可行性。方法 :把HCV复合多表位抗原基因PCX与Pf复合基因AB克隆到带CMV启动子的真核表达载体pcDNA3中 ,构建真核表达载体pcDNA3 CAB ,肌肉注射免疫小鼠及家兔 ,检测其诱发特异性免疫应答水平及安全性。结果 :小鼠及家兔分别于免疫后第 6周及第 8周可检测到抗GZ PCX抗体 ,于第 10周达最高 ,滴度分别为 1:40 0及 1:3 2 0 0 ,但持续时间较短 ;免疫血清可识别Pf抗原 ;免疫动物还可产生针对GZ PCX融合蛋白的迟发性超敏反应 ;免疫后小鼠体重正常 ,肝脾脏未见明显肿大 ,具有良好的安全性。结论 :HCV Pf双价多表位DNA抗原基因在小鼠及家兔中可诱发特异性免疫应答 ,但抗体的持续时间较短。
Objective: To investigate the feasibility of combined DNA vaccine of Hepatitis C virus (HCV) and Plasmodium falciparum (Pf). Methods: The PCX and Pf complex genes of HCV multi-epitope antigen gene were cloned into the eukaryotic expression vector pcDNA3 with CMV promoter. The eukaryotic expression vector pcDNA3 CAB was constructed and injected into mice and rabbits intramuscularly to detect the induced Specific immune response levels and safety. RESULTS: Anti-GZ PCX antibodies were detected in mice and rabbits at 6 weeks and 8 weeks after immunization, reaching the highest at 10 weeks with titers of 1:40 0 and 1: 320, respectively The duration of immunization was short, immune serum could recognize Pf antigen, the immunized animals could also produce delayed hypersensitivity reaction to GZ PCX fusion protein, the body weight of mice immunized was normal, no obvious enlargement of liver and spleen, good safety . CONCLUSION: The HCV Pf bivalent multi-epitope DNA antigen can induce specific immune responses in mice and rabbits, but the antibody duration is short.