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目的 探讨辐射对培养小鼠骨髓基质细胞内NF κB的影响。方法 采用免疫组化、Westernblot及凝胶电泳迁移率实验。结果 免疫组化方法显示正常骨髓基质细胞细胞核的周围表达低水平的NF κB ,8 0Gy60 Coγ射线损伤后 1h即见其表达较正常显著升高并出现明显的核移位 ,4h达高峰 ,6h有所回落 ,8h恢复正常 ;Westernblot显示照射后骨髓基质细胞NF κB的蛋白表达量较正常明显升高 ,8h仍然未恢复正常 ;凝胶电泳迁移率实验发现正常骨髓基质细胞内存在低水平活性NF κB ,8 0Gy60 Coγ射线损伤后 1h即见其活性明显升高 ,4h达高峰 ,8h恢复正常。结论8 0Gyγ射 线损伤后培养小鼠骨髓基质细胞内NF κB的表达升高并激活 ,这可能与骨髓微环境内基质细胞的抗辐射能力及造血功能的恢复有关。
Objective To investigate the effects of radiation on NF κB in cultured mouse bone marrow stromal cells. Methods Immunohistochemistry, Western blot and gel electrophoretic mobility shift assay were used. Results Immunohistochemical staining showed that the expression of NFκB was low in the normal nuclei of the bone marrow stromal cells. The expression of NFκB was markedly increased and markedly nuclear translocated at 1h after 80 Gy 60 Co γ-ray injury, reaching the peak at 4h, Western blot showed that the protein expression of NF-κB in bone marrow stromal cells was significantly higher than that in normal cells and still not returned to normal after 8 hours. The electrophoretic mobility shift assay showed that there was a low level of active NF κB in normal bone marrow stromal cells , 8Gy60Coγ ray 1h after injury that was significantly increased activity, peaked at 4h, 8h returned to normal. Conclusion The expression of NF-κB in bone marrow stromal cells cultured with 0Gy γ-rays is increased and activated, which may be related to the anti-radiation ability of stromal cells in the bone marrow microenvironment and the recovery of hematopoietic function.