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植物名称:金鱼草(Antirrhinum majus)别名龙口花。材料类别:实验材料为橙黄色和红色矮茎、粉红色的中茎。选用杂种F_1后代种苗的第二年萌发的幼芽,用70％的酒精和0.1％的升汞进行表面消毒,切取1cm的小段作为外植体,接种在MS基本培养基上。培养条件:诱导丛生芽的基本培养基为MS培养基。激素处理:(1)BA0.1～2.0mg/L(单位下同)+IBA0.1(BA0.1,0.5,1.0,2.0);(2)BA2.0+NAA0.1。生根培养基:(1)1/2MS+NAA0.02;(2)1/5MS+NAA0.02～0.1。温度为25±2℃,光照度1500lx,每日光照14～16小时。
Plant Name: Snapdragon (Antirrhinum majus) alias Longkou flowers. Material Category: Experimental materials are orange and red short stems, pink stems. The second year sprouting seedlings of hybrid F_1 progeny were germinated with 70% alcohol and 0.1% mercuric chloride for surface disinfection. A 1 cm short section was cut out as explants and inoculated on MS basal medium. Culture conditions: The basic medium for inducing the cluster buds was MS medium. Hormone treatment: (1) BA0.1 ~ 2.0mg / L (unit same below) + IBA0.1 (BA0.1,0.5,1.0,2.0); (2) BA2.0 + NAA0.1. Rooting medium: (1) 1 / 2MS + NAA0.02; (2) 1 / 5MS + NAA0.02 ~ 0.1. Temperature 25 ± 2 ℃, light intensity 1500lx, daily light 14 to 16 hours.