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背景:维生素D受体基因在内切酶BsmⅠ,ApaⅠ,TaqⅠ作用下,呈限制性内切酶片段长度多态性,并且与骨密度密切相关熏而骨密度的变化对骨质疏松起着重要作用,但维生素D受体基因多态性与骨密度、骨质疏松相关性尚无定论。目的:分析在中国汉族、维吾尔族、哈萨克族、蒙古族绝经后妇女中与骨密度密切相关的维生素D受体基因多态性分布规律。设计:对比观察。单位:解放军总医院老年医学研究所。对象:选择2002-01/2003-12在解放军总医院进行健康体检的汉族绝经后妇女179名,平均年龄穴59±3雪岁。选择2001-01/2003-12于解放军兰州军区乌鲁木齐总医院老年科进行健康体检的维吾尔族、哈萨克族、蒙古族绝经后妇女者122,63,112名,平均年龄分别为(56±4),(55±3),(57±3)岁。均对检测项目知情同意。方法:应用聚合酶链反应限制性片段长度多态性技术确定维生素D受体基因BsmⅠ基因型,分析汉族、维吾尔族、哈萨克族和蒙古族绝经后妇女维生素D受体基因BsmⅠ多态性分布频率,并与已知的美国、澳大利亚、法国和日本相应数据进行比较。计数资料差异比较采用χ2检验。主要观察指标:汉族、维吾尔族、哈萨克族和蒙古族绝经后妇女维生素D受体基因BsmⅠ多态性分布频率,以及该分布特点与美国、澳大利亚、法国和日本相应数据比较结果。结果:汉族、维吾尔族、哈萨克族和蒙古族绝经后妇女维生素D受体基因bb基因型频率分别为90.5%,69.67%,38.1%和50%,BB基因型频率分别为0,4.1%,6.35%和4.46%,汉族与维吾尔族、哈萨克族和蒙古族维生素D受体基因型频率分布比较,差异明显穴P<0.01雪。哈萨克族与欧美人种比较熏维生素D受体基因型差异不明显,与日本、韩国人种差异明显(P<0.01)。结论:中国汉族与维吾尔族、哈萨克族和蒙古族绝经后妇女维生素D受体基因型多态性具明显种族差异性;哈萨克族维生素D受体基因型频率分布接近欧美人种,与日本、韩国人种差异明显。
BACKGROUND: Vitamin D receptor gene is restricted by restriction enzyme BsmⅠ, ApaⅠ, TaqⅠ and is closely related to bone mineral density. The change of bone mineral density plays an important role in osteoporosis Role, but the relationship between vitamin D receptor gene polymorphism and bone mineral density, osteoporosis is not conclusive. OBJECTIVE: To analyze the distribution of vitamin D receptor gene polymorphism closely related to bone mineral density in Chinese Han, Uygur, Kazak and Mongolian postmenopausal women. Design: comparative observation. Unit: Institute of Geriatrics, PLA General Hospital. PARTICIPANTS: A total of 179 Han Chinese postmenopausal women were selected for health examination at PLA General Hospital from January 2002 to December 2003, with an average age of 59 ± 3 years old. A total of 122,63,112 Uighur, Kazak and Mongolian postmenopausal women were enrolled in the Department of Geriatrics, Urumqi General Hospital of PLA, Lanzhou Military Command from January 2001 to December 2003, with mean age of (56 ± 4) and (55 ± 3), (57 ± 3) years old. All informed consent of test items. Methods: BsmⅠ genotypes of vitamin D receptor gene were determined by polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP). The frequency of Bsm Ⅰ gene polymorphism was analyzed in Han, Uighur, Kazak and Mongolian postmenopausal women And compared with the known data of the United States, Australia, France and Japan. Count data differences were compared using χ2 test. MAIN OUTCOME MEASURES: The distribution frequency of Bsm Ⅰ polymorphism of vitamin D receptor gene in Han, Uygur, Kazak and Mongolian postmenopausal women was compared with the corresponding data of the United States, Australia, France and Japan. Results: The frequencies of bb genotypes of vitamin D receptor gene in Han, Uygur, Kazak and Mongolian women were 90.5%, 69.67%, 38.1% and 50%, respectively. The frequencies of BB genotypes were 0, 4.1% and 6.35 % And 4.46% respectively. The frequencies of vitamin D receptor genotypes between Han and Uygur, Kazak and Mongolians were significantly different (P <0.01 snow). There was no significant difference in the genotypes of vitamin D receptor among Kazak ethnic groups in Europe and the United States, with significant differences between Japan and South Korea (P <0.01). Conclusion: The genotype differences of vitamin D receptor genotypes between Chinese Han and Uygur, Kazak and Mongolian postmenopausal women have obvious ethnic differences. The frequency distribution of vitamin D receptor in Kazak is close to those in Europe and the United States, Ethnic differences are obvious.